观赏凤梨高效离体快繁影响因素的研究

Influencing factors of efficient and rapid propagation in vitro of Bromeliads

以侧芽为外植体材料,对其组织培养的各个阶段进行了研究。结果表明:品种对外植体的污染率、褐变率、芽诱导率及丛生芽增殖倍数均有显著影响;外源激素的种类和浓度在芽诱导分化、增殖、生根等各个阶段都是主要的影响因素;外植体在1/2MS+NAA0.2mg·L^(-1)+6-BA2mg·L^(-1)上,48d后芽开始分化,诱导率为40%,平均芽分化数为6个,诱导分化效果最好;芽增殖培养阶段,若培养基中仅含6-BA,且浓度在0~4mg·L^(-1)之间,芽增殖倍数随其浓度增大而增大;若培养基中仅含NAA,且其浓度为0.2mg·L-1时,增殖倍数最大;培养基中同时添加6-BA和NAA,比单独添加6-BA或NAA时,芽的增殖效果好,且在6-BA3mg·L^(-1)+NAA0.5mg·L^(-1)的MS培养基上,G.dissitisflora和G.‘Claret’增殖倍数最大,分别为5.24和3.84;在任何相同的培养基上,G.dissitisflora的增殖倍数显著高于G.‘Claret’;小苗在含有NAA0.5mg·L^(-1)+IBA0·5mg·L^(-1)的生根培养基上生长,生根率可达91.03%,平均根数为5.3条/株,生根效果较好。

This paper, every stage was studied by using lateral buds as explants. The results showed that the variety had remarkable influence on the contamination rate and the browning rate of explants,and the induction rate and the proliferation multiple of tufty buds. The variety and concentration of exogenous hormone were the major influencing factors in every stage such as the bud induction, proliferation and the root induction. In the bud induction stage, it got the best result of induction differentiation,which occured after 48 days with 40% induction rate and average six buds per explant when they were cultured on 1/2MS basal media supplemented with NAA 0.2 mg · L^-1 and 6-BA 2 mg · L^-1. In the bud proliferation stage, when the concentration of 6-BA solely supplemented in the media between 0-4 mg · L^-1 was higher,the proliferation multiple was higher. When the concentration of NAA solely supplemented in the media was 0.2 mg · L^-1 , the proliferation multiple was the highest. It got better proliferation on the media supplemented with both 6-BA and NAA than that on the media supplemented with 6-BA or NAA solely. On lVlb media supplemented with 3 mg · L^-1 6-BA and 0. 5 mg· L^-1 NAA, the proliferation multiple of G. dissitisflora and G. ＇Claret＇ both got to the top,which was 5.24,3.84 respectively. On any the same media, the proliferation multiple of G. dissitisflora was remarkable higher than that of G. ＇Claret＇. It was better to root when the plantlets grew on 1/ 2MS media containing 0. 5 mg · L^-1 NAA and 0. 5 mg · L^-1 IBA,and the rooting rate could reach 91.03%, and the average number of the roots was 5.3 piece per plant.