FUT4过表达促进胚胎细胞与子宫内膜细胞的黏附

Overexpression of Fucosyltransferase Ⅳ Promotes Embryonic Cells to Adhere to Uterine Cells

胚胎与子宫内膜上皮细胞之间的黏附是胚胎成功植入的关键.岩藻糖基转移酶Ⅳ(FUT4)对胚胎细胞与子宫内膜细胞黏附的影响未见报道.本研究以人子宫内膜细胞(HEC-1A)和胚胎细胞(JAR)为体外着床模型,观察上调HEC-1A细胞中FUT4表达对JAR细胞与HEC-1A细胞黏附的影响.RT-PCR和免疫细胞化学检测结果显示,FUT4过表达增加HEC-1A细胞中FUT4基因及蛋白的表达;免疫细胞化学及Western印迹结果表明,上调HEC-1A细胞中FUT4增加细胞表面LeY的合成;细胞黏附实验结果显示,与未转染组相比较,FUT4过表达增加了JAR细胞与HEC-1A细胞的黏附率.本研究证明,FUT4过表达可以增加细胞表面LeY寡糖抗原的合成,从而促进胚胎细胞与子宫内膜细胞的黏附.

The adhesion of embryonic cells to the uterine epithelial cells is critical for the successful implantation,but the role of fucosyltransferase Ⅳ（FUT4） on embryo adhesion to uterine epithelial cells remains unknown.Here,we utilized an in vivo implantation model which was composed of JAR and HEC-1A cells,to study the effect of FUT4 on the adhesion of JAR to HEC-1A cells.By FUT4 expression plasmid transfection,FUT4 mRNA and protein level was increased,and also LeY synthesis increased on HEC-1A cell surface,which enhanced the percent adhesion of JAR to HEC-1A cells.These finding suggested that up-regulation of FUT4 increased the synthesis of LeY on HEC-1A cell surface,and enhanced the adhesion of JAR to HEC-1A cells.