一种大脑皮层神经元特异性标记物的研究

A neuron-specific marker in the cerebral cortex

目的探讨一种新型大脑皮层神经元特异性结合物的特性及应用条件。方法将接有荧光素FITC的肽段Tetl通过免疫荧光技术对体外培养的大鼠大脑皮层神经元进行染色，免疫双标法检测FITC-Tetl特异性标记神经元能力，并对其应用条件进行量化。同时以HEK及C6细胞做为对照。结果FITC-Tetl达到一定浓度（30Ixg／mL）时能特异性显示大脑皮层神经元．其几乎不与胶质细胞、HEK及C6细胞结合。过高（100μg／mL）或过低（10μg/mL）浓度均不利于神经元标记。结论Tetl能特异性结合大脑皮层神经元，该肽段可能成为一种新型的中枢神经元标记或靶向手段。

Objective To study the characteristics of a new neuron-specific marker in the cerebral cortex and its application method. Methods According to immunofluorescence principles, the neurons in the cerebral cortex cultured in vitro were stained by fluorescein isothiocyanate （FITC） -labeled Tetl; and FITC-Tetl with NSE or GFAP was employed to determine whether the neurons can be marked and whether they can be distinguished from astrocytes; the conditions of application of FITC-Tetl were quantified. The HEK and C6 cell lines were performed the above experiments as controls. Results The final concentration was the key factor, which indicated that when the FITC-Tetl was about 30μg/mL, the neuron marking ability was the best, and the astrocytes, HEK and C6 were not stained. Neither high （100 μg/mL） or low （10 μg/mL） levels of FITC-Tetl were suitable for staining neurons. Conclusion Tetl can specifically combine with the neuron in the cerebral cortex, which may make it to be a new staining method or a targeting molecule to neurons in the central nervous system.