含氮类培养基对海洋微藻Isochrysis Zhanjiangensis油脂与碳水化合物积累的影响

Effects of Nitrogen-containing Media on the Accumulation of Lipid and Carbohydrate in Marine Microalgae Isochrysis zhanjiangensis

考察了8种含氮培养基对湛江等鞭金藻(I.zhanjiangensis)生长、PSⅡ活性、油脂及碳水化合物积累的影响。结果显示,当培养基中氮浓度为1.5 g/L,藻细胞的总脂肪含量和产量分别达到最高值为39.8%和0.92 g/L,碳水化合物的含量为最低11.6%;而当培养基中氮浓度为0.016 g/L,藻细胞的总脂肪含量和产量分别达到最低值为21.1%和0.16 g/L。而此时总碳水化合物含量最高达到44.4%。同时线性拟合方程的结果表明培养基中NO3-的浓度与藻细胞的总脂含量呈较好的正相关性。因此,通过研究不同含氮水平的培养基实现了脂肪或碳水化合物产量的调控。

Effects of eight media on the growth,PSⅡ activity,lipids and carbohydrate accumulation of a marine microalgae Isochrysis zhanjiangensis were investigated.When the nitrogen concentration in the medium is 1.5 g/L,the highest lipid content of 39.8 % and the maximum lipid yield of 0.92 g/L were achieved,while the carbohydrate content of 11.6 % was the lowest.When the nitrogen concentration in the medium is 0.016 g/L,the lowest biomass of 0.74 g/L and the lowest lipid content of 21.1 % were obtained.However,the highest carbohydrate content of 44.4 % was obtained.Based on the result of linear fitting equation,the concentration of NO3-in the medium has a good positive correlation with total lipid content of algal cells.Therefore,the regulation in the yield of lipids and carbohydrate is realized by studying different nitrogen-level media.