二次心理应激干预对特种兵血清蛋白质谱变化的影响

Effects of repeated psychological stress training on the spectrum of serum protein expression in special troops

目的探讨重复心理应激干预对特种作战部队新兵血清蛋白质谱表达的影响。方法将96名某部特种作战部队新兵随机均分为单纯心理训练组、循环心理训练组和对照组,分别训练4周后,参加为期1d的高强度模拟防暴演习,并于演习结束后第3天,再次参加未被预先告知的高强度模拟防暴演习。于两次演习结束后4h内抽取3组新兵血清,采用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术结合蛋白质芯片检测血清蛋白表达谱的变化并进行统计学分析。结果方差分析结果显示,首次演习后3组血清中质荷比(M/Z)为6417.8、9134.2、15 171.9、14 972.7D的4种蛋白质相对含量有显著差异(P<0.05),且循环训练组4种蛋白表达均显著增高;重复演习后4种蛋白质相对含量也呈明显升高趋势(P<0.05),特别是对照组与首次演习后比较上升明显(P<0.05)。结论心理训练可使应激后血清中低表达的蛋白明显上调。重复性高强度的抗应激训练可迅速提高机体的心理抗应激能力。

Objective To investigate the effects of repeated psychological stress training on the serum protein expression in soldiers under mental stress.Methods Ninety-six male commando soldiers were randomly assigned into the common psychological training group,the circulation psychological training group and the control group（32 each）.After a 4-week training,all the soldiers were instructed to attend an one-day high-intensity simulated anti-riot exercise,and 3 days later attended another unannounced high-intensity simulated anti-riot exercise.Blood samples were collected from all the soldiers within 4 hours after each exercise,and the changes in serum protein expression were determined and statistically analyzed by using surface-enhanced laser desorption/ionization time of flight mass spectrometry（SELDI-TOF-MS） combined with ProteinChip technology.Results The variance analysis showed that significant differences existed among the three groups（P0.05） in the relative contents of proteins with M/Z values of 6417.8,9134.2,15171.9 and 14972.7D after the first anti-riot exercise,and the relative contents of all the above mentioned proteins increased in the circulatory psychological training group;meanwhile,markedly increasing trends of the relative contents of all the proteins were observed in the three groups after the second anti-riot exercise（P0.05）,and in control group the relative contents of the 4 above mentioned proteins were significantly higher than those after the first anti-riot exercise.Conclusion Psychological training may up-regulate the expression of serum proteins that are down-regulated after stress,and the repeated high-intensity mental training can rapidly improve the soldiers＇ ability to counteract stress.