CT/MRI双模态造影剂的制备和表征

Synthesis and characterization of dual modality contrast agent for CT/MRI imaging

目的:合成用于CT/MRI双模态造影的水包油型纳米乳液,并对其进行表征。方法:用共沉淀法合成油酸包裹的Fe3O4纳米颗粒,然后将其分散到市售的碘化油注射液中,借助纳米乳液的合成方法合成出CT/MRI双模态造影剂,并用透射电镜(TEM)、动态光散射(DLS)、水平磁场作用下乳滴的组装、CT和MRI等方法进行表征。结果:(1)TEM结果显示,乳滴的直径约为100 nm,DLS测得其水动力尺寸平均为81.7 nm;(2)将乳液置于水平磁场作用下验证了Fe3O4纳米颗粒确实存在于乳滴内;(3)CT、MRI等分析手段显示了该乳液的CT值为292.6 HU(碘含量为24 mg.ml-1),弛豫率为65.7 mmol.L-1.s-1。结论:CT/MRI双模态造影的水包油型纳米乳液具有良好的CT和MRI造影增强效果,有望成为一种性能优异的CT/MRI双模态造影剂。

Objective： To synthesis a kind of oil-in-water nanoemulsion for dual modal CT/MRI imaging and characterize it.Methods： Using coprecipitation method to synthesis oleic acid coated Fe3O4 nanoparticles,and dispersed them into iodinated oil injection.CT/MRI dual modality contrast agent was prepared on the basis of nanoemulsion formation,which was characterized by TEM,DLS,assembly of nanodroplets under a horizontal magnetic field,CT and MRI.Results：（1） TEM results revealed that the nanoemulsion had a size of 100 nm.DLS study showed the hydrodynamic mean diameter was 81.7 nm,approximately.（2） Placing the nanoemulsion under a horizontal magnetic field showed Fe3O4 nanoparticles existed in the droplets.（3） CT and MRI measurements showed the CT value was 292.6 HU（iodine concentration： 24 mg·ml-1）,and the relaxibility value（r2） of the nanoemulsion was 65.7 mmol·L-1·s-1.Conclusion： This kind of oil-in-water nanoemulsion enhances prominently CT and MRI contrast,which is expected to become an excellent dual modality contrast agent for CT/MRI imaging.