胃肠癌虚实辨证与Th1/Th2细胞因子关系的研究

The Relationship Between Traditional Chinese Medicine Symptoms Differentiation and Th1/Th2 Cytokines Level in Gastrointestinal Cancer

目的研究胃肠癌虚实证型与Th1/Th2细胞因子平衡的关系,筛选胃肠癌虚实证型辨证客观化指标。方法选择胃肠癌100例,其中实证组58例,虚证组42例。另选择40例健康人作为对照组。采用酶联免疫方法(ELISA)检测各组外周血白细胞介素(IL)-2、-4、-6、-10和干扰素(IFNγ)-水平。结果胃肠癌虚实证组均表现出Th1/Th2失衡,Th2优势免疫,且虚证组较实证组表现更为显著,差异有统计学意义(P<0.05或<0.01)。结论胃肠癌虚实证型与细胞因子Th1/Th2有关,Th1/Th2水平可以作为胃肠癌中医辨证客观量化的筛选指标。

Objective To study the relationship of Th1/Th2 cytokines level and patients with gastrointestinal cancer including deficiency and excess symptom,and to search the objective index of the deficiency and excess symptoms.Methods The 100 patients with gastrointestinal cancer were used in this test.These patients were divided into two groups：58 cases of the excess symptom group and the 42 cases of deficiency symptom group.Another 40 healthy people were used as the contrasting group.Several experimental indexes of blood were examined using ELISA,such as interleukin（IL）-2,-4,-6,-10 and interferon（IFN）-γ.Results The imbalance of Th1/Th2 type cytokines and the higher levels of Th2 type cytokines were expressed in the deficiency and excess symptom groups.Compared with the excess symptom group,the imbalance was remarkably statistic differences in the deficiency symptom group（P〈0.05 or 〈0.01）.Conclusion Th1/Th2 type cytokines have close correlation with gastrointestinal cancer including deficiency and excess symptoms,and the level of Th1/Th2 can be used as objective index of the deficiency and excess symptoms in gastrointestinal cancer.