高效液相色谱-串联质谱法测定水产品中黄曲霉毒素G_2、G_1、B_2、B_1

Determination of Aflatoxin G_2,G_1,B_2,B_1 in Aquatic Products with HPLC-MS/MS

建立了用高效液相色谱-串联质谱法(HPLC-MS/MS)测定水产品中黄曲霉毒素G2、G1、B2、B1残留量的方法。84%甲醇水溶液提取水产品中4种黄曲霉毒素,正己烷脱脂,HLB固相萃取柱净化。采用电喷雾电离,正离子扫描,选择多反应检测模式(MRM)监测,外标法定量。该法对4种黄曲霉毒素标准曲线的线性回归系数均在0.99以上,黄曲霉毒素G1、B1方法定量限为0.5μg/kg,G2、B2方法定量限为0.3μg/kg。4种黄曲霉毒素的回收率为56%～80%,相对标准偏差1.58%～14.4%。该法灵敏,结果可靠,可用于水产品中黄曲霉毒素的测定。

The method for determining aflatoxin G2,G1,B2,and B1 by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was developed.The samples were extracted by 84% methanol,degreased with hexane and purified with HLB solid-phase extraction column.In addition,with electrospray ionization in positive mode,the samples was monitored with multiple reaction monitoring（MRM） and quantified them with external standard method.For the 4 aflatoxins,it showed good linear regression coeffcient in the standard curve（all0.99）,the limits of detection for aflatoxin G1,B1 were 0.5 μg/kg,and the limits of detection for aflatoxin G2,B2 were 0.3 μg/kg.The average recovery was 56%~80%,and the relative standard deviation was 1.58%~14.4%.The method,was sensitive and reliable,and can be applied for the determination of aflatoxins in aquatic products.