摘要
利用热回流法和超声波法提取细果角茴香中的总黄酮,结合NaNO2-Al(NO3)3-NaOH体系分光光度法测定细果角茴香中的总黄酮含量,并对细果角茴香中总黄酮的提取方法进行优化。结果表明:利用超声波法提取细果角茴香中的总黄酮效果较好;且在甲醇浓度为70%、料液比为1∶20、超声波提取2次、提取时间分别为2.0 h和1.5 h的条件下提取效果最好,测得总黄酮的含量为15.44 mg/g。NaNO2-Al(NO3)3-NaOH体系分光光度法操作简捷,测定结果准确可靠。
Total flavonoids in H. leptocarpum was extracted by hot reflux method and ultrasonic extraction method, and its content was determined by NaNO2-Al （NO3 ）3-NaOH spectrometry. The results showed that ultrasonic method was more suitable than hot reflux method, and the optimum extraction condition was 70% methanol solution, solid-liquid ratio 1：20 （W/V）, ultrasonic treatment twice with the time of 2. 0 h and 1.5 h. Total flavonoids content in H. leptocarpum was about 15.44 mg/g. NaNO2-Al （NO3 ） 3-NaOH spectrometry method was easy to operate and the results were accurate.
出处
《河北农业科学》
2011年第6期106-108,共3页
Journal of Hebei Agricultural Sciences
基金
青海省重点科技项目(2010-Z-719)
关键词
细果角茴香
总黄酮
提取
含量测定
Hypecoum leptocarpum Hook. f. et Thorns.
Total flavonoids
Extraction
Content determination
