额尔敦-乌日勒对动脉粥样硬化家兔血清中一氧化氮含量和抗氧化酶活性的影响

Effect of Eerdun-wurile on the serum nitric oxide and anti-oxidant enzymes activities of rabbits with atherosclerosis

目的观察额尔敦-乌日勒对动脉粥样硬化（AS）家兔血清中一氧化氮（NO）含量和抗氧化酶活性的影响。方法采用喂饲高脂饲料构建家兔AS模型，将36只家兔完全随机分为4组，每组9只。正常对照组喂普通饲料，其他3组喂高脂饲料。正常组与模型组分别灌胃蒸馏水，辛伐他汀组给予辛伐他汀5ms／（kg·d），额尔敦-乌日勒组给予额尔敦一乌日勒0．4g,／（kg·d），连续90d。检测家兔血清中NO含量，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的活性和丙二醛（MDA）含量，并且测定血清TC、TG、LDL—C、HDL—C的含量，计算各组兔主动脉粥样硬化斑块面积比。结果与模型组相比，额尔敦-乌13勒能提高As家兔血清中NO[（231．26±44．92）μmol／L]含量，同时增强SOD[（57．81±7．65）kU／L]、CAT[（25．71±4．60）kU／L]和GSH-Px[（515．55±81．36）kU／L]的活性（P〈0．05），同时降低MDA[（22．27±11．43）μmo]／L]的含量（P〈0．05）。并且降低血清，TC、TG、LDL-C水平和主动脉斑块面积比（P〈0．05）。结论额尔敦-乌日勒可通过增加血清中NO含量保护血管内皮功能；通过增强机体抗氧化酶的活性，提高抗氧化能力以及通过调节血脂而起到抗AS作用。

Objective To investigate the effects of Eerdun-wurile on the serum nitric oxide （NO） and antioxidant enzymes activities of rabbits with atherosclerosis. Methods An atherosclerotic rabbit model was established by feeding high fat diet. The 36 rabbits were randomly divided into 4 groups. Control group was fed with normal diet, the other 3 groups were fed high fat diet. The normal group and model group were administered distilled water. Positive control group received simvastatin 5 mg/（ kg · d）, the Eerdun-wurile-treated group were given the Eerdunwurile 0.4 g/（kg · d） continuous 90 days. The NO[ （231.26 ±44.92） μmol/L] and the activities of superoxide dismutase（SOD） [ （57.81 ± 7.65 ） U/roll , catalase （CAT） [ （25.71 ± 4.60） U/roll and glutathione peroxides （GSH-Px） （515.55 ± 81.36） U/ml, as well as and the contents of malonaldehyde （MDA） [ （ 22.27 ± 11.43 ） nmol/ml] in serum were measured. The contents of serum total cholesterol （TC）, Triglyceride （TG）, Low-density lipoprotein cholesterol （LDL-C）, High density lipoprotein cholesterol（HDL-C） of rabbits in each of the groups were determined, and the area of rabbit aorta suffering from atherosclerosis plaque were measured. Results Compared with model group, Eerdun-wurile could increase serum NO content and SOD, CAT and GSH-Px activity（P 〈0.05） and reduce the content of MDA in atherosclerosis rabbits （P 〈 0.05 ） and the serum contents of TC, TG and LDL-C, as well as the area of lipid-plaque aorta endothelium of rabbits （P 〈 0.05 ）. Conclusion Eerdun-wurile can protect vascular endothelial function by increasing levels of serum NO, improve the body＇s antioxidant capacity by enhancing the body＇s antioxidant enzyme activity, modulate the serum lipid level and prevent the development of atheroosclerosis in the model.