小鼠感染曼氏裂头蚴后组织病理学改变动态观察

Histopathological dynamic observation on mice infected by experimental Sparganum mansoni

目的动态观察小鼠感染曼氏裂头蚴后的组织病理学改变,了解曼氏裂头蚴对组织的损害。方法从黑斑蛙体内检获曼氏裂头蚴,口服法以3条/只感染昆明小鼠,于感染后第2～10周分别处死小鼠进行组织病理学观察。结果在感染小鼠皮下、肌肉、腹腔等处发现裂头蚴,主要位于皮下肌肉组织。组织病理学观察:肉眼观察,寄生处的组织有弥漫性淤血斑点,囊包块形成;HE染色发现病变部位脂肪组织及横纹肌组织变性、坏死,纤维组织增生并包绕脂肪组织,少部分血管纤维素样坏死等。病变部位早期以中性粒细胞浸润为主的急性炎症,随着病程的进展,局部组织的损伤程度不断加重,炎症范围不断扩大,并出现以淋巴细胞为主的慢性炎症表现。结论小鼠感染曼氏裂头蚴后寄生于多组织器官,其中以皮下肌肉处多见。曼氏裂头蚴寄生处组织随病程进展出现不同程度的以急慢性炎症反应为主的病理损伤。

Objective To observe dynamically the histopathological changes of mice infected with Sparganum mansoni.Methods The mice were infected orally by Sparganum mansoni collected from Rana nigromaculatus（3 strips/mouse）,and 2-10 weeks later,the mice were sacrificed for histopathological observation.Results Sparganum mansoni was found in the skin,muscle,and abdominal cavity of mice.There were diffuse congested spots and capsule formation.Fat tissue appeared severe degeneration and necrosis,and fibrous tissue appeared moderate proliferation of enveloping the adipose tissue,with a large number of neutrophilic mainly acute and chronic inflammatory cell infiltration and more abscess formation,and so on.Conclusions Sparganum mansoni parasitizes mainly in subcutaneous and muscle of mice.The organizations of mice infected with Sparganum mansoni would appear different degrees of histopathological changes.