抗盐碱转基因大豆对根际与非根际土壤氨氧化古菌多样性的影响

Effects of Salinization Resistence Transgenic Soybeans(SRTS) on the Diversity of Ammonia Oxidizing Archaea(AOA) in Rhizosphere Soil and Non-rhizosphere Soil

采用PCR—DGGE技术,研究了抗盐碱转基因大豆(SRTS)对根际与非根际土壤氨氧化古菌(AOA)群落多样性的影响。结果表明,在非根际土壤中,SRTS的氨氧化古菌DGGE条带数、多样性指数显著高于其受体亲本黑农35和其他两种大豆处理,而均匀度指数较低;在根际土壤中,SRTS的DGGE条带数和多样性指数均高于其受体亲本,但并不显著,其均匀度指数则显著高于其他处理;每种大豆自身根际与非根际比较显示,SRTS非根际氨氧化古菌DGGE条带数、多样性指数明显高于根际,均匀度指数却低于根际,而其受体亲本与其他两个处理反之。聚类分析结果表明,SRTS的DGGE带谱与其他大豆处理差异较大,且自身非根际与根际处理差异显著,与其受体亲本黑农35相似性很低。测序结果表明,在SRTS处理中特有条带12、15和优势条带13、14均属于Uncultured crenarchaeote。在盐碱土壤生态系统中,SRTS提高了非根际土壤氨氧化古菌群落的多样性,但对根际土壤中氨氧化古菌的群落多样性有一定的抑制作用。

The use and development of genetically modified soybeans has been a topic of considerable public debate in recent years.The majority of studies addressing potential risks of soybeans cultivation have addressed only aboveground effects.But,recent methodological advances in soil microbial have allowed research focus to move underground to try to gain knowledge of soybeans-driven effects on the microbial communities and processes in soil system.In order to deeply understand the effect of salinization resistence transgenic soybeans（SRTS）on the diversity of rhizosphere ammonia oxidizing archae（AOA） and non-rhizosphere AOA in saline-alkali soil system,PCR-DGGE cloning was used.The main conclusions were shown as follow：The results of DGGE fingerprint showed that the AOA bands＇ number and the diversity indexes of SRTS in rhizosphere soil and non-rhizosphere soil were all higher than its recipient parent HeiNong-35,but it was not significant in rhizosphere soil treatment,and the AOA diversity indexes in non-rhizosphere soil of SRTS were significantly higher compared with its rhizosphere soil treatment,while the recipient parent HeiNong-35 and other two treatments were completely opposite.Cluster analysis demonstrated that fingerprint of SRTS was different from other treatments,and its non-rhizosphere and rhizosphere soil treatments were different,which indicated that SRTS might change the AOA communities in the soil.The experimental results of DGGE-cloning showed the sequences of SRTS unique band 12 and 15,prevailing bands 13 and 14 were all belong to Uncultured crenarchaeote.The study revealed that the SRTS treatment increased the communities＇ diversity of AOA in rhizosphere soil and brought an inhibiting effect on the communities＇diversity of AOA in non-rhizosphere soil.