摘要
目的了解河北省婴幼儿腹泻患者轮状病毒的感染特点及基因型别的变迁情况。方法2007—2010年在河北卢龙县医院和卢龙县妇幼保健院收集5岁以下腹泻住院病例1643例的粪便标本，采用酶联免疫吸附试验（ELISA）检测轮状病毒抗原，阳性标本用多重反转录．聚合酶链反应（RT-PCR）进行基因分型。结果1643例标本中801例轮状病毒抗原阳性，阳性率为48．75％。对轮状病毒抗原阳性标本进行G／P分型，2007—2010年G3型所占比例波动于48．59％-69．92％，G1型由2007年的10．59％下降至2010年的5．79％，G9型则由2007年的2．12％上升至2010年的19．74％。P分型以P[8]最为常见，G／P组合以G3P[8]为主。结论轮状病毒是婴幼儿腹泻的主要病原，G3P[8]为河北省主要流行株，G9型有上升趋势。
Objective To investigate the infection characteristics of group A rotavirus in children with diarrhea in Hebei. Methods Stool specimens were collected from 1643 children （less than 5 years old） with diarrhea in Lulong county hospital, Lulong county maternal and duild health hospital from 2007 to 2010. The specimens were detected by ELISA, and the positive samples were genotyped using multiple reverse transcriptase-polymerase chain reaction. Results A total of 801 positive cases were detected among 1643 patients with diarrhea, with the positive rate of 48.75%. All the positive specimens were genotyped for G/P. G3 serotype ranged from 48.59% to 69.92%, 2007-2010. The proportion of G1 dropped from 10. 59% in 2007 to 5.79% in 2010. However, The proportion of G9 rose from 2.12% in 2007 to 19.74% in 2010. P[8 ]was the most common P genotype. G3P[8 ] ranked first in combination of G/P. Conclusion Rotavirus is the main pathogen of infantile diarrhea. G3P[8 ] is the major epidemic strains and the G9 serotype has a rising trend.
出处
《国际病毒学杂志》
2011年第5期153-156,共4页
International Journal of Virology
