锦灯笼果实总黄酮提取工艺

Extraction Technology for Total Flavonoids from Fruits of Physalis alkekengi

目的:研究锦灯笼果实总黄酮的最佳提取工艺。方法:在单因素基础上,通过正交试验考察乙醇体积分数、料液比、提取时间以及提取次数4个因素对锦灯笼果实总黄酮提取量的影响,用紫外-可见分光光度计对锦灯笼果实总黄酮含量进行测定,得到最佳提取工艺。结果:锦灯笼果实总黄酮最佳提取工艺为乙醇体积分数60%、料液比1∶30、提取时间30 min、提取3次,平均提取量2.00 mg.g-1。结论:乙醇超声法可以用于锦灯笼果实总黄酮的提取,其中料液比对总黄酮提取量的影响最大。

Objective：To study on optimum extraction technology for total flavonoids from fruits of Physalis alkekengi.Method：Based on single factor test,investigated influence of four factors to extract of total flavonoids from P.alkekengi by orthogonal design test.The four factors were concentration of ethanol、ratio of solid to liquid、extraction time and extraction times.Got optimal extraction technology by determined the content of total flavonoids from fruits of P.alkekengi UV-visible spectrophotometer.Result：The optimum extraction technology was as follows：extracted 3 times wtih 30 times amount of 60% ethanol for 30 minutes each times.The average extract of total flavonoids was 2.00 mg·g-1.Conclusion：Total flavonoids from fruits of P.alkekengi can be extracted with ethanol by ultrasonic wave.Ratio of liquid to solid was the most significant factor.