摘要
目的:建立血浆中脱水穿心莲内酯的分析方法,测定脱水穿心莲内酯血浆蛋白结合率。方法:采用超滤法和高效液相法测定脱水穿心莲内酯在BSA、正常人血浆、大鼠血浆中的蛋白结合率。结果:脱水穿心莲内酯与BSA、正常人血浆、大鼠血浆中的蛋白结合率分别为(71.50±1.50)%,(79.91±2.51)%,(82.41±2.05)%。结论:脱水穿心莲内酯与血浆具有中等强度结合。
Objective:To study protein binding percentage of dehydroandrographolide in different plasma samples.Method: The protein binding rates of dehydroandrographolide in BSA plasma samples,human plasma samples and rat plasma samples were determined by ultrafiltration and HPLC.Result: The plasma protein binding rate of dehydroandrographolide with BSA,human plasma,rat plasma were(71.50±1.50)%,(79.91±2.51)%,(82.41±2.05)% respectively.Conclusion: The binding of dehydroandrographolide with plasma is middle degree.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第21期57-59,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30701097)
广东省自然科学基金项目(9151052005000003)
关键词
脱水穿心莲内酯
血浆蛋白结合率
超滤法
dehydroandrographolide
plasma protein binding percentage
ultrafiltration