超滤法测定脱水穿心莲内酯的血浆蛋白结合率

Protein Binding Percentage of Dehydroandrographolide in Different Plasma Samples by Ultrafiltration

目的:建立血浆中脱水穿心莲内酯的分析方法,测定脱水穿心莲内酯血浆蛋白结合率。方法:采用超滤法和高效液相法测定脱水穿心莲内酯在BSA、正常人血浆、大鼠血浆中的蛋白结合率。结果:脱水穿心莲内酯与BSA、正常人血浆、大鼠血浆中的蛋白结合率分别为(71.50±1.50)%,(79.91±2.51)%,(82.41±2.05)%。结论:脱水穿心莲内酯与血浆具有中等强度结合。

Objective：To study protein binding percentage of dehydroandrographolide in different plasma samples.Method： The protein binding rates of dehydroandrographolide in BSA plasma samples,human plasma samples and rat plasma samples were determined by ultrafiltration and HPLC.Result： The plasma protein binding rate of dehydroandrographolide with BSA,human plasma,rat plasma were（71.50±1.50）%,（79.91±2.51）%,（82.41±2.05）% respectively.Conclusion： The binding of dehydroandrographolide with plasma is middle degree.