HPLC同时测定灯盏细辛注射液中6种主要成分的含量

Simultaneous Determination of Six Major Compounds in Dengzhanxixin Injection by HPLC

目的:建立HPLC同时测定灯盏细辛注射液中绿原酸、咖啡酸、1,3-O-二咖啡酰奎宁酸、灯盏花乙素、3,5-二-O-咖啡酰奎宁酸、4,5-二-O-咖啡酰奎宁酸含量的方法。方法:Agilent Zorbax SB-C18(4.6 mm×150 mm,5μm)色谱柱,流动相乙腈-0.1%甲酸水溶液,梯度洗脱,柱温30℃,检测波长327 nm,流速1.0 mL.min-1,进样量20μL。结果:绿原酸、咖啡酸、1,3-O-二咖啡酰奎宁酸、灯盏花乙素、3,5-二-O-咖啡酰奎宁酸、4,5-二-O-咖啡酰奎宁酸的线性范围分别为0.079～5.065(r=0.999 9),0.080～5.120(r=0.999 9),0.074～4.750(r=0.999 9),0.391～25.050(r=0.999 9),0.038～2.423(r=0.999 9),0.487～7.792 mg.L-1(r=0.999 8),加样回收率分别为96.72%(RSD 1.76%),96.64%(RSD 1.69%),101.81%(RSD 1.81%),98.16%(RSD 2.22%),99.02%(RSD 2.57%),97.02%(RSD 1.52%)。结论:方法简便、快捷,准确、重复性好,可为灯盏细辛注射液提供质量控制依据。

Objective：To establish an HPLC method for determination of six major compounds in Dengzhanxixin injection,including chlorogenic acid,caffeic acid,1,3-O-dicaffeoylquinic acid,scutellarin,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid.Method： The analysis was carried on a Zorbax SB-C18（4.6 mm×150 mm,5 μm） column.The mobile phase was composed of acetonitrile-0.1% formic acid with gradient elution.The flow rate was 1.0 mL·min-1.The detection wavelength was set at 327 nm.The column temperature was at 30 ℃,The injection volume was 20 μL.Result： The linear ranges of six compounds were as follows： 0.079-5.065 mg·L-1（r=0.999 9） for chlorogenic acid,0.080-5.120 mg·L-1（r=0.999 9） for caffeic acid,0.074-4.750 mg·L-1（r=0.999 9） for 1,3-O-dicaffeoylquinic,0.391-25.050 mg·L-1（r=0.999 9） for scutellarin,0.038-2.423 mg·L-1（r=0.999 9） for 3,5-dicaffeoylquinic acid,and 0.487-7.792 mg·L-1（r=0.999 8） for 4,5-dicaffeoylquinic acid.The average recoveries were 96.72%（RSD 1.76%）,96.64%（RSD 1.69%）,101.81%（RSD 1.81%）,98.16%（RSD 2.22%）,99.02%（RSD 2.57%）,97.02%（RSD 1.52%） respectively.Conclusion： The method is convenient,rapid and accurate with good reproducibility and could be used for the quality control of Dengzhanxixin injection.