超微六味地黄汤对老年痴呆大鼠认知功能和脑组织碱性成纤维生长因子表达的影响

Study on Effect of Ultra-powder Liuwei Dihuang Decoction on Recognize and bFGF in Alzheimers Disease Rats bFGF

目的:探讨超微六味地黄汤对老年痴呆(AD)模型大鼠学习记忆功能及碱性成纤维生长因子(bFGF)表达的影响,阐明超微六味地黄汤益智健脑的机制。方法:采用β-淀粉样蛋白(Aβ1-40)脑内注射建立AD模型,将动物随机分为正常组、假手术组、模型组、中药超微六味地黄汤10 g.kg-1组,给予不同处理,于造模后5,30 d处死动物,评价动物的认知功能,RT-PCR和ELISA检测bFGF表达水平。结果:造模后动物逃避潜伏期明显延长,治疗后中药组与模型组相比,逃避潜伏期明显缩短,差异均有统计学意义(P<0.05);超微六味地黄汤组与假手术组比较,差异无统计学意义。正常大鼠脑组织有低水平bFGF基因和蛋白表达,造模后模型组和中药组表达增强,模型组、超微六味地黄汤10 g.kg-1组与正常组比较差异有统计学意义(P<0.05),但两组间差异无统计学意义;治疗后超微六味地黄汤组bFGF水平维持在相对较高的水平,而模型组基本恢复至正常组水平,超微六味地黄汤组与模型组、正常组比较差异均有统计学意义(P<0.05)。结论:Aβ1-40脑内注射后动物认知功能下降,超微六味地黄汤能促进其认知功能恢复,增强AD大鼠bFGF表达,是其益智健脑的可能机制。

Objective：To explore the effects of ultra-powder Liuwei Dihuang on recognition and basic fibroblast grouth factor（bFGF） in Alzheimers disease rats,and the mechanism of ultra-powder Liuwei Dihuang promoting recognition.Methold：The rat model of Alzheimer was established by Injection of Aβ1-40,rats were randomly divided into normal group,sham group,model group,Chinese medicines group,then at 5 d and 30 d rats were executed to evaluate the recognizing function,bFGF was assayed by ELISA and RT-PCR.Result： After modeling,rat escape latency was significantly longer,there was significant difference between the model group and Liuwei Dihuang group at 30 day（P〈0.05）;there was not significant differences between sham-operated group and Liuwei Dihuang group.In the normal and sham-operated group rat brain had a low level bFGF,it was increased in model group and the Chinese medicines group at 30 day,there was significant difference between the model group and Liuwei Dihuang group at 30 day point（P〈0.05）;there was not significant differences between sham-operated group and Liuwei Dihuang group.Conclusion： Rat recognition was declined after injection of Aβ1-40 intracerebral,ultra-power Liuweidihuang can promote recognitive function,can enhance bFGF expression in AD rat,which may be possible mechanism of yizhijiannao.