摘要
目的:探讨肝复康胶囊抑制人肝癌细胞系HepG2增殖的作用机制。方法:采用MTT比色法测定肝复康胶囊对人肝癌细胞系HepG2细胞增殖的影响;用流式细胞仪分析细胞周期、细胞凋亡,分析各时期细胞周期的变化;用免疫细胞化学方法检测药物作用后诱导凋亡蛋白Omi/HtrA2和casepase-3的表达。结果:肝复康胶囊具有较好的抑制HepG2细胞增生的作用,呈现出时间-剂量依赖性;应用肝复康胶囊后HepG2细胞G2期比例升高,G1期和S期细胞比例降低,并使细胞凋亡率升高。结论:肝复康胶囊能够抑制人肝癌细胞系HepG2增殖,促进其凋亡。
Objective：To investigate the mechanism of action of Ganfukang capsule to human hepatocellular carcinoma cell line HepG2.Method： The effect of cell proliferation of Ganfukang capsule to human hepatocellular carcinoma cell line HepG2 was determined by MTT colorimetric.The cell cycle,cell apoptosis was analyzed by flow cytometry.Detect induction apoptosis protein Omi/HtrA2 and casepase-3 expression after the action of drug was observed by immunocytochemical method.Result： Ganfukang capsule had good inhibitory effect on HepG2 cell proliferation,showing a time-dose dependent.Application of Ganfukang capsule increased the proportion of HepG2 cells in G2 phase,reduced the proportion of G1 phase cells and S phase cells,and made the apoptosis rate increased.Conclusion： Ganfukang capsule can inhibit proliferation of hepatoma cell line HepG2,and promote their apoptosis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第21期165-168,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
肝复康胶囊
肝癌细胞
流式细胞术
细胞周期
细胞凋亡
Ganfukang capsule
hepatoma carcinoma cell
flow cytometry
cell cycle
cell apoptosis
