4组造模复合因素致肺阳虚小鼠模型的比较研究

Research on Mice Lung-Yang Deficiency Models Induced by Four Different Multi-factors

目的:根据"形寒饮冷则伤肺""劳倦内伤而耗阳气",对烟熏、冰水游泳、服用冰水、服用冰他巴唑溶液等造模因素进行组合,比较4组造模复合因素对肺阳虚证小鼠模型的影响。方法:ICR小鼠,随机分为4个造模组,造模10 d。观测小鼠一般体征、抓力、自主活动,测定肺组织和血清丙二醛(MDA),、超氧化物歧化酶(SOD),计算心、肝、脾、肺、肾等脏器指数。结果:4个模型组小鼠均出现体重增长缓慢,自主活动减少,抓力降低,心、肺指数增加。其中3因素组合对体征以及肺组织和血清MDA,SOD的影响,优于2因素组合;复合造模因素中,增加冰泳(烟熏+冰泳、烟熏+冰泳+服用冰水、烟熏+冰泳+服冰他巴唑)优于服用冰水(烟熏+服用冰水),抓力下降、心肺指数升高更明显;烟熏+冰泳+服用冰水可能是最佳造模复合因素。结论:采用烟熏、冰水游泳、服用冰水、服冰他巴唑等2因素或3因素组合造模,小鼠均表现出不同程度的肺阳虚症状。其中3因素(烟熏+冰泳+服用冰水)造模更接近中医临床,这可能是形寒、劳倦与饮冷共同使然。

Objective： According to the traditional Chinese medicine （ TCM） theory that lung injury was resulted from chilled body shape and cold drink and＂Yang ＂Deficiency was caused by over-strain and internal injury＂,we combined two or three of four Lung-Yang Deficiency factors to compare the effects of these different combinations in. mice Lung-Yang Deficiency models. The four factors were termed as smoking in the special box,swimming in ice water,drinking ice water,and taking the methimazole ice solution. Method： Seventy ICR mice were randomly divided into four model groups and normal group. Each group with 14 animals was treated for 10 days. We observed the general physical signs （ grip strength and self-activity） ,measured MDA and SOD activityof lung tissue and serum,and calculated the organ indexes. Result： The body weight growth decreased,the self-activity and grip strength reduction,and the heart and lung indexes increase were observed in all model groups. Inaddition,the effects of three-factor combination groups on physical signs as well as MDA and SOD of lung tissueand serum were better than which of the two-factor combination groups,resulting in that swimming in ice waterwas more effective than drinking ice water. These results showed that the combination of smoking,swimming inice water,and drinking ice water was the best method to induce Lung-Yang Deficiency. Conclusion： Differentcombinations of four Lung-Yang Deficiency factors indicated different effects. Among these combinations,the three-factor combination （ smoking ＋ swimming in ice water ＋ drinking ice water） was cinsistent with TCM theory about Lung-Yang Deficiency,which may be induced by several factors such as chilled body shape,cold drinks and over-strain.