复方隔山消颗粒对胃肠运动的影响及其机制

Mechanism and Effect of Compound Geshanxiao Granules on Gastrointestinal Motility

目的:探讨复方隔山消颗粒对胃肠运动的影响及其机制。方法:将实验动物随机分为正常对照组、模型对照组、西沙必利(小鼠0.003 g.kg-1,大鼠0.001 5 g.kg-1)组和高、中、低隔山消颗粒(小鼠:10,5,2.5 g.kg-1,大鼠:5,2.5,1.25 g.kg-1)组。按20 mL.kg-1剂量给药6 d后,用硫酸阿托品抑制小鼠小肠推进运动,测定各组小鼠的小肠推进率;以夹尾刺激法制造大鼠功能性消化不良(FD)模型,按10 mL.kg-1剂量给药7 d,测定各组大鼠胃黏膜一氧化氮(NO)含量和血中胃肠激素水平。结果:西沙必利组和高、中、低隔山消颗粒组小鼠小肠推进率均显著高于模型对照组,西沙必利组和高、中、低隔山消颗粒组大鼠胃黏膜NO含量均明显低于FD模型对照组,西沙必利组和高、中隔山消颗粒组大鼠血清胃泌素(GAS)和血浆胃动素(MTL)含量均明显高于FD模型对照组(P<0.05或P<0.01)。结论:复方隔山消颗粒能促进胃肠运动,其机制可能与其降低胃黏膜NO含量,提高血清GAS和血浆MTL水平有关。

Objective：To discuss mechanism and effect of Compound Geshanxiao granules on gastrointestinal motility.Method： Mouse intestinal propulsive activity was inhibited by atropine to observe the movement of small intestine.The rat functional dyspepsia model was established by clipping tail stimulation.The contents of nitrogen monoxidum（NO） in gastric mucous membrane,the level of rat blood gastrin（GAS） and motilin（MTL） were measured.Result： Compound Geshanxiao granules could apparently increase intestinal propulsive activity in mice,and decrease the contents of NO in gastric mucous membrane,meanwhile increase the contents of GAS in serum and MTL in plasma of functional dyspepsia model in rats.Conclusion： Compound Geshanxiao granules can improve intestinal propulsive activity,the mechanism may be related to decreasing the contents of NO in gastric mucous membrane and elevating the level of rat GAS in serum and MTL in plasma.