加味四逆散对肝纤维化大鼠肝组织Ⅳ型胶原和TGFβ_1 mRNA表达的影响

Effects of Jiawei Sini San on Expression of Collagen Ⅳ and Transforming Growth Factor-beta 1 of Hepatic Fibrosis in Rats

目的:观察加味四逆散对肝脏Ⅳ型胶原(C0L-Ⅳ)和转化生长因子β1(TGFβ1)mRNA的影响,探讨其抗肝纤维化的作用机制。方法:将50只动物购回后随机分为4组,除空白对照组10只外,其余40只均用二甲基亚硝胺(DMN,用生理盐水稀释至5 g.L-1)以10 mL.kg-1体重ip,每日1次,每周连续3 d,连续4周的方法制备大鼠肝纤维化模型,加味四逆散按生药量以35 g.kg-1ig给药,采用实时定量PCR技术检测各组大鼠肝组织Ⅳ型胶原和TGFβ1 mRNA表达的水平。结果:加味四逆散组大鼠肝组织C0L-ⅣmRNA为5.136±1.32,TGFβ1 mRNA为4.839±0.48,与病理模型组(C0L-ⅣmRNA 10.369±1.69,TGFβ1mRNA 6.307±0.85)比较有显著性差异(P<0.05)。结论:加味四逆散能够抑制肝纤维化大鼠肝组织C0L-Ⅳ和TGFβ1 mRNA表达,从而发挥其抗肝纤维化的作用。

Objective：The mechanism of Jiawei Sini San（JWSNS） in treating hepatic fibrosis was rexplored and studied by observing the expression of collagen Ⅳ and transforming growth factor-beta（TGFβ1）mRNA in liver.Method：Fifty animals were randomly divided into 4 groups,in addition to 10 in the control group,the remaining 40 were treated with dimethylnitrosamine（DMN,diluted with saline to 5 g·L-1） to 10 mL·kg-1 ip,once daily for 3 d of each week,which lasted 4 weeks.The groups of treatment were fed with JWSNS gavage.Collagen Ⅳ and TGF-β1 were detected in liver samples by RT-PCR.Result：CoL-Ⅳand mRNA of Liver tissue in JWSNS group was 5.136 ± 1.32,TGFβ1 mRNA was 4.839±0.48,Compared with the pathological model group（CoL-Ⅳ mRNA 10.369±1.69,TGF β1 mRNA 6.307±0.85） the result were significantly different（P〈0.05）.Conclusion：The mechanism of treating hepatic fibrosis with JWSNS appears to be due to the enhancement of degradiation of Collagen Ⅳ and TGF-β1.JWSNS can treat hepatic fibrosis effectively.