生宝颗粒对免疫功能低下小鼠免疫功能的影响

Effect of Shengbao Granula on Immunological Function in Mice with Immunological Deficiency

目的:观察生宝颗粒对免疫低下小鼠免疫功能的影响。方法:采用炭末廓清法,T淋巴细胞酯酶染色率法,2,4-二硝基氯苯所致小鼠皮肤迟发超敏反应模型以及鸡红细胞所致小鼠溶血素抗体生成模型,观察生宝颗粒对免疫低下小鼠免疫功能的影响。结果:生宝颗粒7.50,3.75 g.kg-1剂量ig 14 d能够显著提高ip醋酸泼尼松龙所致免疫低下小鼠的炭末廓清指数K值,7.50 g.kg-1剂量同时能够显著提高其脾脏指数,表明能够提高非特异性免疫功能;生宝颗粒7.50,3.75 g.kg-1剂量ig7 d能够显著提高sc氢化可的松所致免疫低下小鼠的外周血T淋巴细胞百分率,ig 13 d能够显著提高ip环磷酰胺所致免疫低下小鼠的皮肤迟发超敏反应,表明能够提高细胞免疫功能;但生宝颗粒7.50,3.75 g.kg-1剂量ig 10 d均不能显著提高sc环磷酰胺所致免疫低下小鼠的溶血素抗体生成,表明对体液免疫功能无显著影响。结论:生宝颗粒能够显著提高免疫低下小鼠的非特异性免疫功能和细胞免疫功能。

Objective：To study the effect of Shengbao granula on immunological function in mice with immunological deficiency.Method： Carbon clearance test,T lymphocyte esterase conversion rate test,2,4-dinitrofluorobenzene-induced delayed type hypersensitivity test（DTH）,and hemolysin antibody formation induced by chick erythrocyte were employed to evaluate the effectsa of shengbao granula on immunological function.Result： Shengbao granula（7.50,3.75 g·kg-1）group could increase the K value and the weight index of spleen in d14 after administration（7.50,3.75 g·kg-1）group increased peripheral T lymphocyte proliferation in d7,enhanced murine ear swelling in d13,and could not increased hemolysin antibody in d10.Conclusion：Shengbao granula significantly improves nonspecific and cellular immunity function in mice with immunological deficiency.