糖平煎对2型糖尿病大鼠炎症细胞因子的干预作用

Intervention Effects of Tangpingjian on Inflammatory Cytokines in Rats with Diabetes Mellitus Type 2

目的:观察糖平煎对2型糖尿病(T2DM)大鼠胰岛素抵抗(IR)和血清脂联素(Adip)、瘦素(leptin)、肿瘤坏死因子-α(TNF-α)的影响。方法:采用高糖高脂饲料喂养和小剂量链脲佐菌素(STZ 30 mg.kg-1)腹腔注射相结合的方法复制T2DM大鼠模型,将T2DM模型大鼠随机分为模型对照组、糖平煎大、小剂量组,以及正常对照组,每组10只。糖平煎大剂量组给糖平煎按生药14.4 g.kg-1.d-1ig,糖平煎小剂量组给糖平煎按生药7.2 g.kg-1.d-1ig,其他两组大鼠给等体积蒸馏水,连续处理4周。观察空腹血糖(FBG)、空腹胰岛素(FINS)、TNF-α、脂联素和leptin水平的变化,计算胰岛素敏感性指数(IAI)。结果:糖平煎大、小剂量组均能明显降低T2DM模型大鼠的FBG、血清胰岛素、leptin和TNF-α水平,提高Adip活性和IAI,与模型对照组比较有显著差异(P<0.01,P<0.05)。结论:糖平煎可以改善T2DM大鼠的炎症反应,减轻高胰岛素血症,提高胰岛素敏感性,改善胰岛素抵抗。

Objective：To investigate the effects of Tangpingjian（TPJ） on insulin resistance（IR） and serum Adiponectin（Adip）,leptin,tumor necrosis factor alpha（TNF-α） in type 2 diabetic（T2DM） rats.Method： The type 2 diabetic rats model was established by the injection of small-dose streptozotocin（STZ 30 mg·kg-1） combined with high-energy feeding.The rats were randomly assigned to the model group,TPJ high-dose,TPJ small dose group and normal control groups.Rats in TPJ high-dose group were performed intragastric administration of 14.4 g·kg-1·d-1 crude drug of Tangpingjian,and rats in TPJ small dose group were administrated with 7.2 g·kg-1·d-1 crude drug of Tangpingjian,the other two groups were administrated with distilled water by equal volume.All four groups had been treated for four weeks.Fasting blood-glucose（FBG）,Fasting insulin（FINS）,TNF-α,Adip and Leptin of rats were measured after four weeps.Calculation of insulin sensitivity index was carried out（IAI）.Result： TPJ could decrease FBG,FINS,leptin and TNF-α remarkably,and improve insulin sensitivity index and Adip activity obviously in experimental rats.It has remarkable difference compared with model group（P〈0.01,P〈0.05）.Conclusion： TPJ can improve T2DM rat inflammatory response,reduce hyperinsulinemia,increase the insulin sensitivity,and improve insulin resistance.