hrHPV-DNA和p16^(INK4a)蛋白在宫颈病变中的表达

Expression of hrHPV-DNA and p16^(INK4a) protein in cervical lesions

目的了解hrHPV-DNA载量及p16INK4a蛋白的表达,探讨hrHPV-DNA和p16INK4a蛋白与宫颈病变的关系,为宫颈癌早期诊断以及预测宫颈病变进展提供可靠的检测指标。方法利用第二代杂交捕获法检测hrHPV-DNA载量,采用免疫化学方法检测p16INK4a蛋白,运用检验与Spearman等级相关研究HPV-DNA和p16INK4a与宫颈病变的关系。结果随着活检分级升高,hr HPV阳性率和p16INK4a阳性表达率增高(P<0.05);正常或慢性宫颈炎中hr HPV阳性率、p16INK4a阳性表达率、HPV(+)/P16(+)表达率低于在CIN1、CIN2、CIN3、SCC中的表达(P<0.05);等级相关性分析在HPV(+)/P16(+)与活检分级呈正相关,在HPV(-)/P16(-)与活检分级呈负相关(P<0.05)。结论 hrHPV-DNA和p16INK4a蛋白均可以作为宫颈癌早期诊断的标志物。联合检测hrHPV-DNA和p16INK4a蛋白可以预测宫颈病变的进展。

【Objective】This study was to detect the infection of human papillomavirus（HPV） and the expression of p16INK4a in cervical lesions and to investigate the relevance between HPV and p16INK4a in cervical lesions.【Method】hr HPV DNA was detected by the Digene hybrid capture II system.Immunochemical method was used to detect the expression of p16INK4a.Histopathologic test was performed to identify cervical lesions of all the cases.【Results】hr HPV positive rate and p16INK4a expression increased（P 0.05） with histopathologic diagnosis increasing.The positive rates of hrHPV,p16INK4a,HPV（＋）/P16（＋） in negative or chronic inflammation were statistically lower than that in cervical intraepithelial neoplasia（CIN） 1,CIN2,CIN3 and squamous-cell carcinoma（SCC）（P 0.05）,respectively.The expression of HPV（＋）/p16（＋） was positively correlated with the severity of cervical lesions（P 0.05）,whereas the expression of HPV（-）/p16（-） was negatively correlated with the severity of cervical lesions（P 0.05）.【Conclusions】hr HPV and p16INK4a are useful markers for the early diagnosis of cervical lesions.Combined measurement of hr HPV and p16INK4a can predict the progress of cervical lesions.