摘要
通过在MS培养基中加入抗生素和杀菌剂进行逐步筛选,解决马铃薯脱毒试管苗快繁过程中的污染问题。结果表明:防止马铃薯脱毒试管苗污染的最佳抑菌剂组合MS+50mg/L硫酸庆大霉素+50mg/L氨苄青霉素+50mg/L硫酸链霉素+0.4g/L多菌灵,该组合有效降低了马铃薯脱毒苗细菌和真菌污染,提高了快繁效率。
In order to solve contamination problem of virus-free potato plantlets in rapid propagation,this experiment by joining antibiotics and fungicide in MS medium gradually screened and choosed. The results showed that best antibacterial agent preventing the virus-free potato plantlets contamination, namely, MS＋ 50 mg/L Gentamycin Sulfate＋ 50 mg/L Ampicillin Sodium＋ 50 mg/L Streptomycin Sulfate ＋ 0. 6 g/L carbendazim. Through this way, bacteria and fungi contamination of virus-free potato plantlets were reduced effectively and efficiency of rapid propagation could alse be improved.
出处
《北方园艺》
CAS
北大核心
2011年第20期134-136,共3页
Northern Horticulture
基金
国家“十一五”科技支撑计划资助项目(2009BADC5B04)
关键词
马铃薯脱毒苗
污染
抑菌剂
控制效果
virus-free potato plantlets
contamination
antibacterial agent
control effect
