摘要
采用RT-PCR法克隆了大豆异黄酮生物合成途径中查尔酮合酶(Chalcone synthase,CHS)和查尔酮异构酶(Chalcone Isomerase,CHI)2个关键酶基因,并利用SOE-PCR技术成功将2个基因融合,将其均构建成原核表达载体,在大肠杆菌中进行了初步的表达研究,为进一步研究CHS和CHI 2种关键酶的功能奠定了基础。同时构建了真核表达载体,采用农杆菌转化法侵染大豆子叶节,获得了转基因抗性植株。
Chalcone Synthase（CHS）and Chalcone Isomerase（CHI）are two key enzymes in soybean isoflavones biosynthesis pathway,the cDNAs of CHS and CHI were cloned using RT-PCR method and these two genes were fused using SOE-PCR,then constructed E.coli expression vectors to study the expression.The eukaryocyte expression vectors were also constructed and transferred into soybean cotyledonary nodes using Agrobacterium-mediated transformation,and then obtained the resistant transgenic plants.
出处
《大豆科学》
CAS
CSCD
北大核心
2011年第5期743-748,共6页
Soybean Science
基金
转基因生物新品种培育重大专项资助项目(2008ZX08004-003)
吉林省科技厅重点资助项目(20080204)
国家自然科学基金资助项目(30971808)
关键词
大豆异黄酮
查尔酮合酶
查尔酮异构酶
表达分析
Soybean isoflavones
Chalcone synthase
Chalcone Isomerase
Expression Analysis
