不同大豆基因型再生性及对农杆菌敏感性的研究

Regeneration and Sensitivity to Agrobacterium of Different Soybean Genotypes

采用农杆菌介导的子叶节遗传转化方法对16个大豆基因型进行转化,以丛生芽分化率和GUS阳性率作为指标,比较不同大豆基因型再生性及对农杆菌敏感性的差异。同时对大豆遗传转化再生过程中的种子萌发所需6-BA浓度、草丁膦(PPT)筛选压力等因素进行研究。结果表明:萌发培养基中添加适宜浓度的6-BA能显著提高丛生芽的分化率;不同大豆品种对PPT的敏感性不同,最适PPT浓度主要集中在5～7 mg.L-1;16个大豆基因型再生性及对农杆菌敏感性差异较大,其中合丰45和东农50的再生率及GUS表达率最高,是适合大豆遗传转化的基因型。

Sixteen soybean genotypes had been transformed by Agrobacterium-cotyledonary node system in this research.Differentiation ratio of multiple shoots and GUS positive rate as indicators,the regeneration of different soybean cultivars and their susceptibility to Agrobacterium tumifaciens were compared.Some factors affecting regeneration and genetic transformation such as 6-BA concentration in germination medium and PPT selection pressure were also studied.The result showed that germination medium supplemented with suitable concentration of 6-BA significantly increased the differentiation rate of buds;the sensitivity of PPT was different with varieties,the optimal concentration of PPT mainly concentrated in 5-7 mg·L-1;Regeneration and sensitivity to Agrobacterium of 16 soybean genotypes were quite different,Hefeng 45 and Dongnong 50 were suitable genotypes for soybean transformation which had the highest regeneration rate and GUS expression.