粉红粘帚菌代谢物对南方根结线虫卵孵化及二龄幼虫的影响(英文)

Effects of Metabolites of Gliocladium roseum on Egg Hatching and Juvenile Mortality of Meloidogyne incognita

粉红粘帚菌(Gliocladium spp.)是一类对植物病原菌具有潜在生物控制作用的真菌,为了探讨粘帚菌对南方根结线虫(Meloidogyne incognita)的抑制作用,实验室条件下研究了粉红粘帚菌挥发性代谢产物和非挥发性代谢产物不同稀释度对南方根结线虫卵孵化及二龄幼虫活性的影响。结果显示非挥发性代谢产物在原液、5倍、10倍、20倍、50倍稀释浓度下,12 d后对南方根结线虫卵孵化的相对抑制率分别为80.4%、12.0%、10.5%、6.7%和5.2%,对二龄幼虫的矫正死亡率分别为32.0%、9.3%、2.0%、0.6%和0.1%,与对照组相比差异显著;挥发性代谢产物对南方根结线虫卵孵化的相对抑制率为22.5%,对二龄幼虫的相对死亡率为17.4%。因此,粉红粘帚菌的非挥发性和挥发性代谢产物对南方根结线虫卵孵化及二龄幼虫活性均有一定的抑制作用。

The filamentous fungi Gliocladium spp.has potential as a biocontrol agent against many pathogens.The objective of this study was to evaluate the effects of ferment filtrate and volatile metabolites of G.roseum on egg hatching and juvenile mortality of Meloidogyne incognita.The nematode egg hatching rates and juvenile activity for treatments with volatile metabolites and with 0×,5×,10×,20× and 40× dilutions of the G.roseum culture filtrate were measured in this test.Results showed that G.roseum ferment filtrate inhibited nematode egg hatching and juvenile mortality by 80.4% and 32.0%,respectively.Dilution of ferment filtrate reduced the inhibition of nematode egg hatching and the juvenile activity.Volatile metabolite of G.roseum also inhibited nematode egg hatching and juvenile activity.G.roseum can be used as a biocontrol agent against plant parasitic nematodes.