经胰腺动脉灌注自体外周血干细胞治疗难治性糖尿病

Perfusing Autologous Blood Stem Cell in Pancreatic artery in Treatment of Refractory Diabetics

目的探讨经胰腺动脉灌注自体外周血干细胞治疗难治型糖尿病可行性、安全性和疗效。方法 5例患者注射G-CSF300ug/天,3～5天后采用MCS+ED方法收集单个核细胞20～50×109,经股动脉插管至脾动脉,用球囊导管临时阻断脾动脉远端后经脾A向胰腺动脉内灌注干细胞悬液。结果 5例患者术后血糖和糖化血红蛋浓度(P值0.003)均下降;胰岛素和C肽水平均有不同提高,与治疗前比较刺激后INS60、HBAlc、C-(P0,60,120,180min)P值<0.05。结论临时阻断脾动脉远端后经脾A向胰腺动脉内灌注干细胞悬液治疗难治型糖尿病可行、安全、有效;技术操作间单。

Objective To probe feasibility, safety and curative effect of perfusing autologous blood stem cell in pancreatic arteries for treating refractory diabetics after blocking distal segment of splenic artery temporarily. Method Five patients were injected G-CSF（300ug/d） subcutaneously for 3 - 5 consecutive days for collecting mononuclear cell about 20 - 50×10^9 using the MCS＋ED blood cell separator. Catheter was insert into splenic artery by seldinger technique, And autologous blood stem cells were perfusedin pancreatic artery by catheter after obstructed distal segment of splenic artery temporarily. Results The serum glucose and HBAlc were decreased, INS and C-P were increased. Conclusion Perfusing Autologous Blood Stem cell in pancreatic artery for treating refractory diabetics after blocking distal segment of splenoartery temporarily is feasible and effective, safe and simple.