40份玉米自交系的ISSR遗传多样性分析

Genetic Relationship of 40 Maize Inbred Lines Based on ISSR

利用ISSR分子标记技术对40份玉米自交系进行亲缘关系分析。从59条ISSR引物中筛选出10条重复性高、多态性好的引物,分别对全部供试材料进行扩增,共扩出90条清晰谱带,其中多态性条带81条,多态性比率为90.00%,表明供试材料基因组DNA的多态性较高。用NTSYSpc-2.10软件中的UPGMA进行聚类分析,40份玉米自交系的遗传相似系数变化范围在0.65～1.00之间,在此基础上构建聚类分析树状图,揭示了玉米各自交系间的亲缘关系。本研究为今后分子水平上玉米优良自交系的改良与选育提供了一定的参考依据。

Genetic relationship of 40 maize inbred lines were analyzed by inter-simple sequence repeat （ISSR） marker.Ten primers,which selected from 59 primers,were used to amplify the maize simple,90 bands were amplified,including 81 polymorphic bands.The percentage of polymorphic bands was 90.00%.The results showed that plenty diversities existed in the maize inbred lines.Cluster analysis by UPGMA indicated that genetic similarity was from 0.65～1.00.The result revealed the genetic relationship between different maise inbred lines.All the result provide reference for to fine varieties improvement and breeding on molecular level.