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延边奶山羊-绵羊体细胞重构胚融合条件的研究 被引量:1

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摘要 采用延边奶山羊耳皮肤成纤维细胞为供核细胞,绵羊卵母细胞为核移植受体,构建了山羊-绵羊异质重构胚,探讨了不同融合电场强度、脉冲次数对重构胚融合率及重构胚体外发育率的影响。结果表明,融合电场强度为1200~1400V/cm、融合脉冲次数为2次,是延边奶山羊-绵羊体细胞克隆的适宜融合条件。
机构地区 延边大学农学院
出处 《江苏农业科学》 CSCD 北大核心 2011年第5期277-278,共2页 Jiangsu Agricultural Sciences
基金 国家自然科学基金(编号:30860184) 公益性行业(农业)科研专项(编号:3-45)
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