60种花卉多酚、黄酮含量及其抗氧化活性

Polyphenols and flavones contents and antioxidant activity in 60 kinds of flowers

为了筛选出抗氧化能力强的花卉品种,对60种花卉植物的50%乙醇提取物进行了多酚、黄酮含量测定,并对提取物进行清除DPPH的试验。结果表明:花卉中含有丰富的多酚和黄酮类物质,多酚含量在70mg/g以上的花卉有芍药花、金盏菊、黄刺玫、美蔷薇、红玫瑰等,黄酮含量较高的花卉有连翘、马蹄莲、山楂、杏、榆叶梅等,含量在7.05～8.33mg/g。不同花卉的醇提物抗氧化活性不同,其中红玫瑰抗氧化活性最强,DPPH清除率达74.37%,其次为月季、牡丹、郁金香等,DPPH清除率为47.78%～66.57%;通过对不同花期多酚、黄酮的测定分析,发现花蕾期大于盛开期,而抗氧化活性在不同花期之间没有一定的规律性。

In order to find out the best flower species of antioxidant activity,polyphenols and flavones content in 50% ethanol extract of 60 kinds of flower plants were determined and DPPH free radical-scavenging test was made.The results showed that flowers were good source of polyphenols and flavones,flowers in which polyphenols content was above 70 mg/g included Paeonia laciflora Pall,Calendula arvensis,R.xanthina Lindl,R.pimpinellifoliascotch rose and Rosa rugosa Thunb.,flowers with higher flavones content included Forsythia suspersa Vahlgolden bells,Zantedeschia aethiopica,Crataegus pinnatifida Bge,P.armeniaca L.and Prunus triloba Lindl.,which ranged from 7.05 to 8.33 mg/g.Ethanol extract in different flowers showed different antioxidant activity.Among them,Rosa rugosa Thunb.was the best,DPPH scavenging rate researched up to 74.37%.Secondly,Rosa chinensis Jacq.,Paeonia suffruticosa Andr.and Tulipa gesneriana L varied from 47.78% to 66.57%.Polyphenols and flavones contents in the bud period were found higher than that in the bloom period based on the determination and analysis at different flowering periods,and the antioxidant activity in different flowering periods showed no regularity.