硫酸镁对瑞芬太尼引起痛觉过敏的影响

Effect of magnesium sulfate on remifentanil-induced hyperalgesia

目的研究大鼠切口痛模型中硫酸镁对瑞芬太尼诱发的痛觉过敏的影响。方法42只雄性SD大鼠，随机均分为七组：切口痛组（I组）、瑞芬太尼组（R组）、切口痛＋硫酸镁组（MgI组）、切口痛＋瑞芬太尼＋硫酸镁（250、500、1000mg／kg）组（MgR1、MgR2、MgR3组）、对照组（C组）。MgI、MgR组均于麻醉前10min腹腔注射硫酸镁稀释液4ml，余组给予相应生理盐水4ml。除C组外，各组均制作切口痛模型。各组均测定并计算出术前24h（T0），术后6、24、48h（T1、T2、T3）大鼠右后爪的热缩足反射阈值（PWTL）及机械缩足反射阈值（PMW）。结果与I组比较，T1～T3时R组PWTL值明显缩短，PMW值明显减少（P〈0．05）；MgI组PWTL值缩短和PMW值减少（P〈0．05）。与R组比较，MgR1、MgR2、MgR组PWTL值缩短和PMW值减少（P〈0.05）。结论硫酸镁可减轻大鼠切口周围组织疼痛，并能缓解由瑞芬太尼诱发的切口周围组织痛觉过敏。

Objective To observe the effect of magnesium sulfate （MgSO4）on remifentanil- induced hyperalgesia in rats model of incision pain. Methods Forty-two male Sprague Dawley （SD） rats were randomly divided into seven groups（n= 6） ： group C （control）, group I （incision pain）, group R （remifentanil ＋ incision pain）, group MgI （incision pain ＋ MgSO4 1 000 mg/kg）, group MgR1 （remifentanil＋incision paint MgSO4 250 mg/kg）, group MgR2 （remifentanil＋ incision paint MgSO4 500 mg/kg）, MgR3 group （ remifentanil ＋ incision pain ＋ MgSO4 1 000 mg/kg）. MgSO4 contained in 4 ml volume was administered intraperitoneally ten minutes before anesthesia in MgSO4 treatment groups, while saline was administered subcutaneously in group C, group I and group R. All groups except group C received incision pain model. Paw Withdrawal Thermal latency （PWTL） and Paw Mechanical Withdrawal Threshold （PMW） were detected on the rats＇ right hind paw at T0 （24 h before operation）, T1 （6 h after operation）, T2 （24 h after operation）, and T3 （48 h after operation） in all groups. Results The PWTL and PMW of group R were significantly shorter at T1 ,T2,T3, when compared with group I （P〈0.05）. The decrement of PMW and PWTL was alleviated by MgSO4 （P 〈0. 05）. The PWTL and PMW of group MgR1, MgR2, and MgR3 were significantly longer when compared with group R （P〈0. 05）. Conclusion MgSO4 can relieve the incision pain and alleviate remifentanil-induced hyperalgesia in rats.