摘要
初步研究了在体外生产猪的原核期胚胎。结果表明:将在体外成熟培养44 h的卵母细胞移植入发情当日的母猪体内,同时进行人工授精,受精后16 h冲出胚胎,获得同期原核期胚胎的效率最高,可以降低多精入卵的发生率。
The author primarily studied the in vitro production of prokaryotic embryos in pig.The results indicated that the following operations could get the highest efficiency of prokaryotic embryos production,and reduce the phenomena of polyspermy： transplanting the oocytes,which have been cultivated in vitro for 44 hours,into the sow＇s oviduct on the day of its oestrus,conducting the artificial insemination at the same time,and rushing out the embryos 16 hours after insemination.
出处
《江西农业学报》
CAS
2011年第9期146-148,共3页
Acta Agriculturae Jiangxi
基金
国家转基因重大专项"优质转基因猪新品种培育"(2008ZX08006-002)
"节粮型高瘦肉率转基因猪新品种培育"(2008ZX08006-003)
湖北省国际科技合作项目"利用siRNA技术培育猪新品种的研究"(2009BFA012)
动物胚胎工程及分子育种湖北省重点实验室开放课题(2011ZD120~129)
关键词
猪
卵母细胞
体外成熟
体内受精
原核
胚胎
Pig
Oocytes
In vitro maturing
Internal fertilization
Prokaryotic stage
Embryo
