波长切换HPLC法同时测定甘草及其炮制品中7个物质的含量

HPLC with switching wavelength simultaneous determination of seven constituents in licorice and itsprocessed products

目的:建立高效液相色谱法测定甘草及炮制品中芹糖基甘草苷、甘草苷、芹糖基异甘草苷、异甘草苷、甘草素、甘草酸、异甘草素的含量。方法:采用Dikma Technologies-C18(200 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.05%磷酸水溶液,梯度洗脱,流速:0.8mL.min-1,检测波长为276 nm(0～16 min)、360 nm(16～24 min)、276 nm(24～28 min)、248 nm(28～38min)、370 nm(38～42 min),柱温30℃。结果:在本文色谱条件下,芹糖基甘草苷、甘草苷、芹糖基异甘草苷、异甘草苷、甘草素、甘草酸、异甘草素的进样量分别在0.056～0.56,0.095～0.95,0.026～0.26,0.015～0.15,0.013～0.13,0.348～3.48,0.003～0.03μg范围内与色谱峰面积呈良好的线性关系;加样回收率(n=6)均在96.1%～98.1%,RSD均小于2.6%。16个来源甘草药材及其4种不同炮制品中7个物质的含量有一定差异。结论:本法快速、准确,重复性好,可更好地控制甘草药材及其炮制品的质量。

Objective：To establish an HPLC method for simultaneous determination of seven constituents （ liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, glycyrrhizic acid, isoliquiritigenin） in licorice and its processed products. Methods：The separation was performed on a Dikma Technologies - C18 BDS （200 mm × 4.6 mm, 5 μm） column with the gradient elution of acetonitrile - water containing 0.05% phosphoric acid at the flow rate of 0.8 mL · min^-1. The column temperature was set at 30 ℃. Detection wavelength was 276 nm in 0 - 16 min,360 nm in 16 -24 min,276 nm in 24 -28 min,248 nm in 28 - 38 min,370 nm in 38 - 42 min. Results： Liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, glycyrrhizic acid, isoliquiritigenin had good linearity in the ranges of 0. 056 -0. 56,0. 095 -0. 95, 0. 026 -0. 26,0. 015 -0. 15,0. 013 -0. 13,0. 348 -3.48, and 0. 003 -0. 03 μg, respectively. The average recoveries of seven constituents were 96. 1% -98.1%, with RSDs≤2. 6%. The contents of seven constituents in licorice from sixteen sources and four processed products had differences. Conclusions：The developed method is rapid, accurate with high repeatability and stability, which is helpful to control the quality of licorice and its processed products .