不同品种芒果总黄酮的抗氧化活性

Antioxidant Activity of Total Flavonoids from Different Mango Varieties

以广西主栽金煌芒果、象牙芒果和台农芒果3个芒果品种为研究对象,测定芒果中总黄酮含量。采用羟自由基(.OH)和超氧阴离子自由基(O-2.)体系、DPPH自由基体系对3个芒果品种总黄酮的抗氧化活性进行研究,并同VC的抗氧化活性进行比较。结果表明:在质量浓度40～200μg/mL范围内,芒果总黄酮和VC对.OH的清除能力从大到小依次为:台农芒果、象牙芒果、金煌芒果、VC;在质量浓度10～200μg/mL范围内,对O-2.清除能力从大到小依次为:VC、台农芒果、象牙芒果、金煌芒果;在质量浓度1～20μg/mL范围内,对DPPH自由基清除能力从大到小依次为:VC、台农芒果、金煌芒果、象牙芒果。3种芒果总黄酮均有良好的抗氧化效果,且与质量浓度呈明显的量效关系;台农芒果总黄酮抗氧化性优于其他两个品种。

Three commercially available mango varieties including Jinhuang mango,Xiangya mango and Tainong mango were determined for the contents of total flavonoids.Meanwhile,the antioxidant activity of these mango varieties was evaluated using hydroxyl,superoxide anion and DPPH radical scavenging systems and compared with that of vitamin C.The results showed in decreasing order of the hydroxyl free radical scavenging activity of total flavonoids in the concentration range of 40-200μg/mL,three commercially available mango varieties and vitamin C could be ranked as follows： Tainong mango,Xiangya mango,Jinhuang mango and vitamin C.In terms of the superoxide anion free radical scavenging activity,the deceasing order was vitamin C,Tainong mango,Xiangya mango and Jinhuang mango in the concentration range of 10-200 μg/mL.In the concentration range of 1-20 μg/mL,vitamin C exhibited the highest scavenging activity against DPPH free radicals,followed by Tainong mango,Jinhuang mango and Xiangya mango.Total flavonoids from all the three mango varieties had obvious antioxidant activity in a dose-dependent manner.Total flavonoids from Tainong mango revealed a better antioxidant capacity than those from other two varieties.