响应曲面法优化植物乳杆菌胞外多糖的醇沉工艺

Optimization of Ethanol Precipitation of Exopolysaccharides Produced by Lactobacillus plantarum 70810 by Response Surface Methodology

利用单因素试验和响应曲面法对植物乳杆菌胞外多糖醇沉工艺参数进行优化研究。结果表明:醇沉时间、95%乙醇添加量、发酵液浓缩倍数与植物乳杆菌胞外多糖提取量存在显著的相关性;胞外多糖醇沉的最佳条件为:醇沉时间12.56h、95%乙醇添加量与发酵液体积比为3.9、发酵液浓缩倍数3.54倍,在此条件下理论预测最大胞外多糖提取量为323.11mg/L,验证实验条件下实际测得最大胞外多糖提取量为320.17mg/L,与预测值的相对误差为0.91%。

The optimal conditions of ethanol precipitation for exopolysaccharides（EPS） produced by Lactobacillus plantarum 70810 were investigated by one-factor-at-a-time experiments and response surface methodology.A mathematical model was developed to reveal the significant effects of each factor and their interactions on the yield of EPS.The optimal parameters for ethanol precipitation of EPS from Lactobacillus plantarum 70810 were 12.56 h precipitation time,3.9：1 ethanol/fermentation supernatant ratio（V/V） and 3.54-fold condensation of fermentation supernatant.Under the optimal conditions,the model-predicted and experimental values of EPS yield were 323.11mg/L and 320.17 mg/L,respectively,with a relative error of 0.91%.