蜂胶不同体积分数乙醇提取物对小鼠肠道麦芽糖酶和蔗糖酶的抑制作用

Inhibitory Effect of Ethanol Extract from Propolis on Maltase and Sucrase in Rat Intestinal Tract

为了研究蜂胶不同体积分数乙醇提取物对餐后血糖的控制作用,采用不同体积分数乙醇对蜂胶进行提取,测定其总黄酮和总多酚含量,并利用高效液相对其成分进行初步分析。并使用体外麦芽糖酶和蔗糖酶抑制模型,研究蜂胶不同体积分数乙醇提取物对来自小鼠肠道麦芽糖酶和蔗糖酶的抑制作用。结果表明:75%乙醇提取物的总黄酮含量最高,达到(697.36±6.15)mg芦丁/g蜂胶提取物,而25%乙醇提取物含有的总多酚含量最高,为(386.50±17.40)mg没食子酸/g提取物;蜂胶75%乙醇提取物对来自小鼠肠道的蔗糖酶半数抑制质量浓度最低,为(32.30±0.60)μg/mL;蜂胶水提取物对小鼠肠道的麦芽糖酶半数抑制质量浓度最低,为(32.60±0.20)μg/mL;与阳性对照组(阿卡波糖)相比,不同体积分数乙醇提取物对来自小鼠肠道蔗糖酶半数抑制质量浓度要小,存在着显著差异(P<0.05)。

In order to explore the control effect of ethanol extract from propolis（EEP） on postprandial blood glucose,propolis was extracted by ethanol with various concentrations.The contents of total flavonoids and total phenols in EEPs were analyzed by high performance liquid chromatography（HPLC）.The inhibitory effect of EEPs on sucrase and maltase in rat intestinal tract was also investigated.The results indicated that EEP extracted by 75% ethanol revealed the highest content of total flavonoids,which was（697.36 ± 6.15） mg rutin/g EEP.However,EEP extracted by 25% ethanol had the highest content of total phenols,which was（386.50 ± 17.40） mg gallic acid/g EEP.Moreover,the EEP extracted by 75% ethanol showed the highest anti-sucrase activity with IC50 of（32.30 ± 0.60） μg/mL.In contrast,the aqueous extract of propolis exhibited the highest anti-maltase activity with IC50 of（32.60 ± 0.20）μg/mL）.Compared with the positive control group,the IC50 of each EEP against sucrase from rat intestinal tract exhibited a significant decrease（P 0.05）.