摘要
目的:研究孕酮(PROG)对大鼠大脑皮质神经元氨基酸受体亚单位GBR1和NR2B表达的影响。方法:不同剂量(1,10,100μmol.L-1)的孕酮处理原代培养的大鼠大脑皮质神经元,采用免疫细胞化学法测定神经元氨基酸受体亚单位GBR1和NR2B表达的变化。结果:与对照组相比,1,10,100μmol.L-1 PROG使GBR1表达分别增加45.9%、172.0%、211.6%(P<0.01),使NR2B表达分别增加42.1%、51.4%、88.4%(P<0.05或P<0.01)。结论:孕酮可增强氨基酸受体亚单位GBR1和NR2B的表达水平。
OBJECTIVE To investigate the effect of progesterone(PROG) on the levels of GBR1 and NR2B expression in primary cultured rat cerebral cortical neurons. METHODS Primary cultured rat cerebral cortical neurons were treated with different concentrations PROG (1, 10, 100 μmol. L-1 ) and the expression of amino acids receptor subunit GBR1 and NR2B were detected by immunocytochemistry. RESULTS Compared with control group, the average optical density of GBRI increased by 45.9%, 172. 0%,211.6% after incubating with PROG,PROG-M,PROG-H (P〈0. 01), the average optical density of NR2B increased by 42. 1%,51.4%,88. 4% after PROG-L,PROG-M,PROG-H treatment(P〈0. 05 or P〈0. 01 )as well. CONCLUSION PROG can increase the expression of neuron receptor subunit GBR1 and NR2B.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第21期1762-1765,共4页
Chinese Journal of Hospital Pharmacy
基金
河北省自然科学基金资助项目(编号:C2005000834)