紫外线诱变纤维素酶高产菌株的筛选及其酶活力

Mutation Screening of High Cellulase-producing Strains by Ultraviolet and Its Enzyme Activity

为筛选产纤维素酶酶活力高的菌株应用于秸秆饲料发酵,利用紫外线诱变对1株产纤维素酶绿色木霉菌进行了试验研究。结果表明:筛选出1株纤维素酶产酶量高且性状稳定的高产菌株ZJUV18,其发酵72h纤维素酶滤纸酶活达68.07U/g,较原始菌株提高4.45倍。

A Trichoderma viride strain was treated with ultraviolet to screen out a high cellulase-producing strain and apply in straw feed fermentation.The results showed that a high cellulase-producing strain with high cellulose yield and stable characteristics,which was named ZJUV18,was screened out.The FPA could be up to 68.07 U/g after fermented for 72 hours,which was 4.45 times the activity of the original strain.