摘要
为给黔北麻羊品种遗传资源的保护及进一步选育提供科学依据,利用BsmAI、MspI、EcoRI、MvaI、XmnI、MnII等6种限制性内切酶对黔北麻羊MSTN基因部分内含子1、2和外显子2进行PCR-RFLP多态性分析。结果表明:在扩增的MSTN基因1 241bp序列中含有BsmAI、EcoRI、MvaI和MnII 4个酶切位点,但均不具有多态性;未发现MspI和XmnI酶切位点。
In order to provide the basic information and scientific proof to breed conservation of genetic resources and further breeding about the Qianbei Ma goat,the polymorphism of intro 1,extro 2 and partial intro 2 in MSTN of Qianbei Ma goat with BsmAI,MspI,EcoRI,MvaI,XmnI,MnI I six kinds of restriction enzymes were studied.The results showed that the sequence of 1 241 bp in MSTN gene contains four restriction enzyme cutting sites which were BsmAI,EcoRI,MvaI and MnI I,but all of them have no polymorphism,while MspI、XmnI restriction sites were not found.
出处
《贵州农业科学》
CAS
北大核心
2011年第10期131-133,共3页
Guizhou Agricultural Sciences
基金
贵州省科学基金项目"贵州山羊肌肉生长抑制素基因多态性与生产性能相关性研究"[黔科合J字(2009)2136]