摘要
目的:研究姜黄素对长期摄入酒精和不同量蛋白质的大鼠胰腺腺泡细胞损伤的保护作用。方法:实验分为5组,即正常对照(正常饲养)、高蛋白、低蛋白、高蛋白+姜黄素、低蛋白+姜黄素(以25%酒精代替饮水自由饮用,高、低蛋白质占总热量供给的32%、6%,喂饲6个月)组。在光镜和电镜下观察大鼠胰腺腺泡细胞结构变化,用比色法检测胰腺组织匀浆淀粉酶和脂肪酶的含量,TUNEL法检测腺泡细胞凋亡情况,免疫组化检测胰腺组织切片中环氧化酶-2(COX-2)的变化。结果:与高、低蛋白组比较,高、低蛋白+姜黄素组大鼠胰腺腺泡细胞髓样结构减少,线粒体肿胀减轻;淀粉酶和脂肪酶含量均显著升高(P<0.05);胰腺腺泡细胞凋亡显著减少(P<0.05);COX-2的表达降低。结论:姜黄素可预防摄入酒精联合过高或过低蛋白质的大鼠胰腺腺泡细胞损伤,延缓酒精性胰腺损伤的进程。
OBJECTIVE: To investigate the protective effects of curcumin on pancreatic acinar cell injury in rats with long-term alcohol intake and protein consumption. METHODS: Wistar rats were divided into 5 groups, i.e. normal control group (the group fed with normal feed), high protein group, low protein group, high protein+curcumin group, low protein+curcumin group (those groups fed with diet containing 25% ethanol instead of drinking water for 6 months). High and low protein accounted for 32% and 6 % of total heat quantity. The structure change of pancreatic acinar cell was observed under light microscope and electron microscope. The contents of amylase and lipase in pancreatic tissue homogenate were determined by colorimetry. Apoptosis and expression of cyclooxygenase-2 (COX-2) in acinar cell were detected by TUNEL and immunohistochemical staining, respectively. RESULTS: Compared with no application of curcumin, myelin figure and enlarged mitochondria were reduced in curcumin treatment groups. Lipase and amylase were increased in curcumin treatment group(P〈0.05). And the expression of COX-2 and apoptosis of pancreatic acinar cell were significantly decreased in curcumin groups (P〈0.05). CONCLUSION: Curcumin is significant in slowing down the progress of alcoholic pancreatic injury and prevent pancreatic acinar cell injury induced by ethanol and high or low protein.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第43期4041-4043,共3页
China Pharmacy
基金
重庆市卫生局医学科研项目(06-2-096)
重庆医科大学校内基金项目(QD200508)
关键词
胰腺炎
酒精性
姜黄素
胰腺腺泡细胞
退行性变
Pancreatitis
Alcoholic
Curcumin
Pancreatic acinar cell
Retrogression