猪EPHA1基因多态性及其与产仔数的关联性分析

Association Analysis between Polymorphisms of EPHA1 Gene and Litter Size in Pigs

研究利用PCR-SSCP技术对大白、长白和杜洛克猪EPHA1基因外显子多态性及其与产仔数的关系进行了研究。结果表明：在第15外显子上检测到一个发生G→C颠换的突变位点,形成3种基因型。对于大白猪,3-6胎的GG型个体的产活仔数（NBA）比CC型个体高出1.66头（P〈0.05）;在所有胎次中,GG型个体的总产仔数（TNB）比CC型个体高出0.2头（P〈0.01）。对于长白猪,第2胎GG型个体的TNB和NBA分别比CC型个体高出1.41头（P〈0.05）和1.46头（P〈0.05）;所有胎次中,GC型个体TNB比CC型个体高出0.67头（P〈0.05）。对于杜洛克猪,3种基因型个体的TNB和NBA差异不显著（P〉0.05）。对于试验大白猪群体而言,该多态位点GG基因型可作为TNB性状的标记基因型。

The polymorphism EPHA1 gene of pig（Large White,Landrace and Duroc） was studied by PCR SSCP,and the association between the polymorphism and litter size was analyzed in order to find the molecular marker being used in marker-assisted selection（MAS） to accelerate the swine breeding progress.There were one polymorphisms occurring in exon15,which change from G to C,with three genotypes（GG,CG and CC）.In Large White,GG genotype had significantly higher（P0.05） NBA than CC genotype in third to sixth parity,and in all parities,GG genotype had greatly significantly higher（P0.01） TNB than CC;In Landrace,GG genotype had significantly higher（P0.05）TNB and NBA than CC genotype in the second parity,and in all parities,GC genotype had significantly higher（P0.05） TNB than CC genotype.In Duroc,there was no significant difference on TNB and NBA.In Large White,the GG genotype was favorable marker genotype for TNB and NBA.