高温胁迫对灰岩皱叶报春生理指标的影响

Effects of High Temperature Stress on Physiological Indicators of Primula forrestii

本研究对灰岩皱叶报春进行30、35、40和45℃24 h的高温胁迫,之后置于室温下恢复12 h,测定叶片的叶绿素、丙二醛、游离脯氨酸、可溶性蛋白、可溶性糖以及相对电导率,以研究灰岩皱叶报春的耐热性。结果表明:随胁迫时间的延长,各温度处理下,叶绿素含量呈先上升后下降趋势,MDA含量和相对电导率持续上升,且随胁迫加剧各指标变化幅度加大。游离脯氨酸、可溶性糖以及可溶性蛋白含量在40和45℃胁迫下先上升后下降,而30和35℃处理下持续上升。灰岩皱叶报春能耐受35℃以下较长时间的高温胁迫,以及40℃下24 h的胁迫,而45℃处理12 h即产生明显伤害,24 h处理后较难恢复。

In order to study the heat resistance,the changes of chlorophyll,malondianldehyde（MDA）,proline,soluble protein,soluble sugar contents in leaves and relative conductance rate of leaves of Primula forrestii with 30,35,40 and 45℃ heat stress for 24 hours,then recovered at room temperature were dtermined.The results showed that,with extending treated time,chlorophyll content first climbed up then went down,while MDA content and relative conductance rate increased all the way and the temperature was higher,the change was more dramatic.Proline,soluble sugar and soluble protein content first grew and then decreased under 40 and 45 ℃ treatment,but they increased steadily during the stress under 30 and 35 ℃.The conclusions were drawn that Primula forrestii could tolerate the heat under 35 ℃ for a relatively long time,40 ℃ for 24 h,but when Primula forrestii was treated under 45 ℃for 12 h,it was seriously hurted and when it was treated under 45 ℃for 24 h it was hard to recover.