比较虚寒证与虚热证模型大鼠肝全基因表达谱的差异

Difference of all gene expression profiles of rat liver between model of deficient cold syndrome and model of deficient heat syndrome

目的采用基因芯片技术研究虚寒证与虚热证大鼠肝全基因表达谱的差异。方法使用中药复方虚寒证、虚热证大鼠模型,基因芯片检测各组大鼠基因表达,筛选差异表达基因,进行基因功能分类注释,荧光定量PCR验证芯片结果。结果虚寒模型组与虚热模型组比较有114条基因差异表达,主要涉及氧化还原酶相关基因。脂代谢相关氧化还原酶基因显著下调,细胞色素P450家族基因显著下调,提示虚寒证模型大鼠脂代谢与生物转化能力的降低。结论虚寒证与虚热证虽然同为虚证,但有"寒证"和"热证"不同的表现,氧化还原酶活性相关基因的异常表达可能是虚寒证与虚热证出现不同症状的物质基础。

Objective To study the difference of all gene expression profiles of rat liver between model of deficient cold syndrome and model of deficient heat syndrome by using gene chip technology. Methods The rat model of deficient cold syndrome or deficient heat syndrome was established through giving TCM compound formulas. The gene expressions in rats of all groups were detected by using gene chips for screening the genes with differential expressions. The genetic function was commented according their classification. The chip results were tested by using fluorescent quantitation polymerase chain reaction （PCR）. Results There were 114 genes with differential expressions in comparison between deficient cold model group and deficient heat model group groups, and they involved mainly the genes related to oxidoreductase activity. Oxidoreductase genes rdlated to fat metabolism and cytochrome P450 genes were down-regulated significantly,which indicated that fat metabolism and bioconversion decreased in rat model of deficient cold syndrome. Conclusion Deficient cold syndrome and deficient heat syndrome, although being deficient syndrome as well, are different in cold and heat symptoms. The abnormal expressions of the genes related to oxidoreductase activity may be the material base of different symptoms in deficient cold syndrome and deficient heat syndrome.