APC和NeuN在肺腺癌A549细胞株的表达

Expression of APC and NeuN in Human Lung Adenocarcinoma Cell Lines A549

目的探讨APC和NeuN在肺腺癌A549细胞株中的表达及意义.方法体外培养肺腺癌A549细胞株,观察细胞形态变化;取第1代培养后1 d、5 d的细胞,用免疫组化检测APC和NeuN在肺腺癌细胞株A549的表达及计处阳性染色细胞的变化.结果 APC和NeuN在体外传代培养的肺腺癌A549细胞株中有表达,且随着细胞的生长,APC的表达减少,NeuN的表达增多.结论 APC可能在其早期形成中就发挥一定的作用;A549、NeuN的表达增A549肺腺癌细胞可能存在向神经细胞分化的潜力.在肺腺癌A549细胞表达减少.

Objective To investigate the expression of APC and NeuN in the lung adenocarcinoma cell lines A549 and its significance.Methods Cell lines A549 were cultured and their morphological changes were observed.After culturing for 1 day and 5 days,expression of APC and NeuN in cells were detected by immunohistochemistry and the change of positive staining cells were counted.Results APC and NeuN expressed in human lung adenocarcinoma cell lines A549 in vitro.In the process of cell growth,the expression of APC reduced,but the expression of NeuN increased.Conclusions The inactivation of APC protein may play a role in early tumor formation.The increased expression of NeuN indicates that lung cancer cells may exist the potential to differentiate into neural cells.