胰岛素信号通路相关因子在2型糖尿病合并骨质疏松大鼠肝组织的表达

Hepatic Gene Expression Involved in Insulin Signaling Pathway in Type 2 Diabetic Osteoporosis Rats

通过高糖高脂饲料联合小剂量链脲佐菌素和去卵巢手术制备2型糖尿病合并骨质疏松大鼠模型,探讨2型糖尿病合并骨质疏松大鼠肝组织胰岛素信号通路相关因子的表达及意义。结果表明:随着时间延长,2型糖尿病合并骨质疏松组(DOVX组)肝组织IGF-1、IRS-1较其他组mRNA及蛋白表达减少,单纯去卵巢组(NOVX组)IGF-1、IRS-1 mRNA及蛋白表达较假手术对照组(NS组)降低;糖尿病组(DS组)IRS-2较NS组mRNA及蛋白表达下降,但NOVX组与NS组IRS-2 mRNA及蛋白表达比较无明显差别。以上结果表明,2型糖尿病合并骨质疏松的发生可能与肝脏胰岛素信号通路受抑制有关。

High sugar and fat feeding, intraperitoneally streptozotocin injection, and ovariectomized surgery were used to set up model of type 2 diabetic osteoporosis rats, to explore hepatic insulin signaling pathway during type 2 diabetic with osteoporosis. The results showed that with 12 week observing, the mRNA and protein expressions of liver IGF-1 and IRS-1 in DOVX group were lower than the other three groups, these expressions in NOVX group were lower than NS group; the mRNA and protein expressions of IRS-2 in DS group were lower than NS group, but no difference found between NOVX and NS groups. In conclusion, type 2 diabetic osteoporosis might be correlated with impaired hepatic insulin signaling pathway.