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IL-8对肺腺癌A549细胞迁移的影响及其机制探讨 被引量：3

Effect and Mechanism of IL-8 on the Cell Migration in Lung Adenocarcinoma A549 Cells
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摘要 探讨IL-8对肺腺癌A549细胞迁移的影响及其可能机制。用MTT法选择了合适的IL-8使用浓度。分别用划痕试验及Transwell试验证明了IL-8可以促进肺腺癌A549细胞的迁移。Westernblot结果表明:(1)IL-8可以促进MMP-2蛋白的表达,而对MMP-9的表达无明显影响;(2)IL-8可促进JNK/SAPK磷酸化蛋白的表达;(3)抑制剂(SP600125)可以阻断IL-8对MMP-2蛋白表达的影响。划痕试验从反面验证了低表达的MMP-2可以抑制A549细胞的迁移。表明IL-8可通过JNK/SAPK信号通路调控MMP-2蛋白的表达,进一步促进肺腺癌A549细胞的迁移。 To investigate the effect and mechanism of IL-8 on the cell migration in lung adenocarcinoma A549 cells, the appropriate concentration of IL-8 treated with cells was chosen by MTT arrays. Wound-healing experiment and Transwell arrays were used to prove that IL-8 can promote the migration of lung adenocarcinoma A549 cells. The results of Western blot showed that： （1） IL-8 can promote the expression of MMP-2 protein, but no significant effect on the expression of MMP-9. （2） IL-8 can promote the expression of p-JNK/SAPK. （3） Inhibitor （SP600125） can block the impacts of IL-8 on the expression of MMP-2. Wound-healing experiment proved that lower expression of MMP-2 can inhibit A549 cells migration. IL-8 can regulate the expression of MMP-2 protein and further promote the migration of lung adenocarcinoma A549 cells through the JNK/SAPK signaling pathway.
作者 杨同宁 于超 李佳佳 朱冰 唐云乔 江跃全
机构地区 重庆医科大学第二附属医院胸心外科 重庆医科大学生命科学研究院 重庆市肿瘤研究所
出处 《中国细胞生物学学报》 CAS CSCD 2011年第10期1102-1108,共7页 Chinese Journal of Cell Biology
基金 重庆市自然科学重点项目(No.2009BA5083) 重庆医科大学重点基金(No.XBED200806)资助项目~~
关键词 IL-8 MMP-2 MMP-9 A549细胞 细胞迁移 IL-8 MMP-2 MMP-9 A549 cells cell migration
分类号 R734.2 [医药卫生—肿瘤]
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参考文献17

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二级参考文献27

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共引文献8

同被引文献42

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引证文献3

二级引证文献10

中国细胞生物学学报
2011年 第10期

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