榄香烯对K562细胞株的体内外抑制作用

In vivo and in vitro anti-tumor effects of elemene on human leukemia cell line K562

目的:探讨榄香烯对慢性粒细胞白血病急变的细胞K562株的体内外作用。方法:不同浓度榄香烯体外作用K562细胞不同时间,CCK-8法检测体外增殖抑制作用,Annexin V/PI双染色法检测细胞凋亡,DAPI染色进行细胞核形态学分析,动物实验观察榄香烯对K562的体内抑制作用。结果:榄香烯对K562细胞生长具有明显的抑制作用,呈量效和时效关系;榄香烯能诱导K562细胞凋亡,呈量效关系;DAPI染色显示榄香烯诱导K562细胞并显示特征性的凋亡细胞核形态;榄香烯明显抑制K562细胞在SCID小鼠体内增殖。结论:榄香烯主要通过诱导细胞凋亡,发挥对K562细胞明显的增殖抑制作用。

Objective： To explore the inhibitory effects of elemene on human chronic myelocytic leukemia cell line K562 in vitro and in vivo.Methods： K562 cells were incubated with different concentrations of elemene.The growth of K562 cells were observed with CCK-8 colorimetric assay,apoptosis rates were detected by flow cytometry using Annexin V-FITC/PI staining,analysis of nuclear morphology were observed with DAPI staining,And its in vivo anti-tumor effect on transplanted K562 cells in SCID Beige mice was detected as well.Results： K562 cells viability in presence of elemene decreased markedly in a dose and time dependent manner.The apoptosis could be induced by elemene and its level increased followed the augmentation of the drug concentration.The characteristic apoptosis nuclei were observed in DAPI staining after elemene treatment.Elemene had a great ability to inhibit the growth of K562 cells in SCID Beige mice.Conclusion： Elemene can significantly inhibit the growth of K562 cells,its function is mainly performed by inducing apoptosis.