沂蒙黑山羊子宫中FSHR和LHR基因在发情周期内的表达规律

MRNA EXPRESSION REGULARITY OF FSHR GENE AND LHR GENE IN UTERUS OF THE ESTROUS CYCLE IN YIMENG BLACK GOAT

为了探讨低繁山羊子宫中FSHR基因和LHR基因的表达变化规律,本研究从沂蒙黑山羊子宫中提取总RNA,使用相同的RT-QPCR方法,分别对处于发情周期不同阶段沂蒙黑山羊子宫各段中FSHR基因和LHR基因在mRNA水平上进行定量分析。结果显示:FSHRmRNA和LHRmRNA在整个发情周期的子宫各段中都有表达。FSHRmRNA的整体表达量水平均高于LHRmRNA的表达量;FSHRmRNA在子宫角发情后期表达量最高,渐低至发情前期最低;子宫肉阜在间情期表达量最高,至发情前期最低,四时期之间差异不显著(P>0.05);子宫颈在发情期表达量最高,间情期表达量最低,与其它时期差异显著(P<0.05)。LHRmRNA在子宫角、子宫肉阜、子宫颈均在间情期表达量最高,且与其它三个时期差异显著(P<0.05)。两基因在子宫体的表达量较低且规律不明显。

To investigate the variation law of mRNA expression levels of FSHR gene and LHR gene in uterus of low productive Goats,total RNA was extracted from the uterus of YiMeng Black goat and Real Time Fluorescence Quantitative PCR method（real-time qRT-PCR） was established so as to analyse mRNA levels of FSHR gene and LHR gene in uterus of four estrous cycle phases in YiMeng Black Goat.The results showed that mRNA of FSHR gene and LHR gene were expressed in the uterus during the four estrous cycle phrases.The expression of FSHmRNA was higher than that of LHRmRNA.In cornua uteri,the expression of FSHmRNA was highest in metestrus and lowest in proestrus;in uteri caruncle the expression of FSHmRNA was the highest in diestrus and lowest in proestrus and there was no obvious difference among the four phases（P0.05）.In cervical,the expression of FSHmRNA was highest in estrus and lowest in diestrus,which is significantly different with other phases（P0.05）.In cornua uteri,uteri caruncle and cervical,the expression of LHRmRNA was highest in diestrus,which is significantly different with the rest phases（P0.05）.In uterine body,the expression of FSHmRNA and LHRmRNA were low and no obvious law was observed.